Mutagenic and Spectroscopic Investigation of pH Dependent CooA DNA Binding

The carbon monoxide (CO) sensing heme protein, CooA, is a transcription factor which exists in several bacteria that utilize CO as an energy source. CooA positively regulates the expression of coo genes in the presence of CO such that the corresponding proteins may metabolize CO. The present studies have yielded the unexpected result that Fe(III) CooA binds DNA tightly at pH \u3c 7, deviating from all previously reported work which indicate that CooA DNA binding is initiated only when the exogenous CO effector reacts with the Fe(II) CooA heme. This observation suggests that the disruption of one or more salt bridges upon effector binding may be a critical feature of the normal CooA activation mechanism. To test this possibility, several protein variants that eliminated a selected salt bridge for the CooA homolog from Rhodospirillum rubrum were prepared via site-directed mutagenesis. Samples of these variant proteins, which were overexpressed in Escherichia coli, were then characterized by spectroscopic methods and functional assays to investigate the impact these mutations had on CooA heme coordination structure and DNA-binding activity. Results of this work are presented in light of the accepted CooA activation mechanism

Systems test facilities existing capabilities compilation

Systems test facilities (STFS) to test total photovoltaic systems and their interfaces are described. The systems development (SD) plan is compilation of existing and planned STFs, as well as subsystem and key component testing facilities. It is recommended that the existing capabilities compilation is annually updated to provide and assessment of the STF activity and to disseminate STF capabilities, status and availability to the photovoltaics program

Test of a NASA blunt body with flares in the JPL 20-inch supersonic and 21-inch hypersonic wind tunnels

Aerodynamic characteristics of flares mounted on blunt bodies at supersonic and hypersonic speed

Consequences of narrow cyclotron emission from Hercules X-1

The implications of the recent observations of a narrow cyclotron line in the hard X-ray spectrum of Hercules X-1 are studied. A Monte Carlo code is used to simulate the X-ray transfer of an intrinsically narrow feature at approximately 56 keV through an opaque, cold magnetospheric shell. The results of this study indicate that if a narrow line can be emitted by the source region, then only about 10% of the photons remain in a narrow feature after scattering through the shell. The remaining photons are scattered into a broad feature (FWHM approximately 30 keV) that peaks near 20 keV. Thus, these calculations indicate that the intrinsic source luminosity of the cyclotron line is at least an order of magnitude greater than the observed luminosity

Field test experience

As a part of the Flat-Plate Solar Array Project (FSA), a field-test program was developed to obtain solar photovoltaic (PV) module performance and endurance data. These data are used to identify the specific characteristics of module designs under various environmental conditions. The information obtained from field testing is useful to all participants in the National Photovoltaics Program, from the research planner to the life-cycle cost analyst

Spittlebug Control in Legumes

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Energy Spectrum Evolution of a Diffuse Field in Elastic Body Caused by Weak Nonlinearity

We study the evolution of diffuse elastodynamic spectral energy density under the influence of weak nonlinearity. It is shown that the rate of change of this quantity is given by a convolution of the linear energy at two frequencies. Quantitative estimates are given for sample aluminum and fused silica blocks of experimental interest.Comment: 9 pages, 3 figures; revised for better presentatio

Static inconsistencies in certain axiomatic higher-order shear deformation theories for beams, plates and shells

AbstractStatic inconsistencies that arise when modelling the flexural behaviour of beams, plates and shells with clamped boundary conditions using a certain class of axiomatic, higher-order shear deformation theory are discussed. The inconsistencies pertain to displacement-based theories that enforce conditions of vanishing shear strain at the top and bottom surfaces a priori. First it is shown that the essential boundary condition of vanishing Kirchhoff rotation perpendicular to an edge (w,x=0 or w,y=0) is physically inaccurate, as the rotation at a clamped edge may in fact be non-zero due to the presence of transverse shear rotation. As a result, the shear force derived from constitutive equations erroneously vanishes at a clamped edge. In effect, this boundary condition overconstrains the structure leading to underpredictions in transverse bending deflection and overpredictions of axial stresses compared to high-fidelity 3D finite element solutions for thick and highly orthotropic plates. Generalised higher-order theories written in the form of a power series, as in Carrera’s Unified Formulation, do not produce this inconsistency. It is shown that the condition of vanishing shear tractions at the top and bottom surfaces need not be applied a priori, as the transverse shear strains inherently vanish if the order of the theory is sufficient to capture all higher-order effects. Finally, the transverse deflection of the generalised higher-order theories is expanded in a power series of a non-dimensional parameter and used to derive a material and geometry dependent shear correction factor that provides more accurate solutions of bending deflection than the classical value of 5/6

An interpretation of photometric parameters of bright desert regions of Mars and their dependence on wave length

Photometric data from the bright desert areas of Mars were used to determine the dependence of the three photometric parameters of the photometric function on wavelength and to provide qualitative predictions about the physical properties of the surface. Knowledge of the parameters allowed the brightness of these areas of Mars to be determined for any scattering geometry in the wavelength range of 0.45 to 0.70 micron. The changes that occur in the photometric parameters due to changes in wavelength were shown to be consistent with their physical interpretations, and the predictions of surface properties were shown to be consistent with conditions expected to exist in these regions of Mars. The photometric function was shown to have potential as a diagnostic tool for the qualitative determination of surface properties, and the consistency of the behavior of the photometric parameters was considered to be support for the validity of the photometric function

Solar pumped laser

A solar pumped laser is described in which the lasant is a gas that will photodissociate and lase when subjected to sunrays. Sunrays are collected and directed onto the gas lasant to cause it to lase. Applications to laser propulsion and laser power transmission are discussed